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KMID : 0616120230540040225
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Korean Journal of Pharmacognosy
2023 Volume.54 No. 4 p.225 ~ p.230
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Antioxidant Activity of Ethyl acetate Fraction of Ixeris chinensis (Thunb.) Nakai in Caenorhabditis elegans
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Choi Ji-Woo
Kim Jun-Hyeong
Ju Nam-Jin
Kim Dae-Keun
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Abstract
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The antioxidant activity of the methanol extract of Ixeris chinensis was examined using a Caenorhabditis elegans model system. The ethyl acetate-soluble fraction (EAf) of the I. chinensis methanol extract exhibited the best DPPH radical scavenging activity. The EAf was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress resistance by using C. elegans along with the active oxygen species (ROS) level. In addition, in order to determine whether the regulation of stress response genes is responsible for the increase of stress resistance of C. elegans treated with the EAf, SOD-3 expression was measured using a transforming strain CF1553. As a result, the EAf increased the SOD and catalase activities and reduced ROS accumulation in a dose-dependent manner, and the CF1553 worm treated with the EAf exhibited higher SOD-3::GFP intensity, compared to the control worm.
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KEYWORD
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Ixeris chinensis, Caenorhabditis elegans, Antioxidant activity
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